The aim of the current study was to investigate negative processing of implicit information relating to self by self-esteem implicit association test in depressed and non-depressed students.17 depressed students and 17 non-depressed students were selected according to a convenience sampling method. Beck depression inventory (BDI-II) and clinical interviews were employed to diagnose depressed individuals. The Beck anxiety inventory (BAI) was conducted to control of participant's anxiety. The data were analyzed using analysis of covariance (ANCOVA).Results revealed that depressed individuals had higher scores in Beck depression inventory (BDI-II) than non-depressed (p = 0.001). It is also a significant difference between depressed and non-depressed individuals (p = 0.003).According to the results of this study, it can be concluded that depressed studentshave lower implicit self-esteem scores than non-depressed, in self-esteem implicit association test (IAT).